验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11416-1-AP targets COX7A2L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | COX7A2L fusion protein Ag1980 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome c oxidase subunit VIIa polypeptide 2 like |
| 别名 | COX7a related protein, COX7A2L, COX7AR, COX7RP, EB1, SIG81 |
| 计算分子量 | 114 aa, 13 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC007095 |
| 基因名称 | COX7A2L |
| Gene ID (NCBI) | 9167 |
| RRID | AB_2245402 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14548 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytochrome c oxidase (COX) is the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. The mitochondrially encoded subunits function in electron transfer, and the nuclear encoded subunits may function in the regulation and assembly of the complex. COX7A2L (cytochrome c oxidase subunit 7A-related protein), also known as COX7AR or COX7RP, is an inner mitochondrial membrane protein. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. Recently it was found that DNA methylation of COX7A2L had a significant association with therapy response in patients with breast cancer. In Alzheimer's disease the expression of COX7A2L was reduced.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COX7A2L antibody 11416-1-AP | Download protocol |
| IHC protocol for COX7A2L antibody 11416-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Supercomplex assembly determines electron flux in the mitochondrial electron transport chain. | ||
EMBO J Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes. | ||
Cell Rep Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics.
| ||
Free Radic Biol Med Cardioprotective strategies preserve the stability of respiratory chain supercomplexes and reduce oxidative stress in reperfused ischemic hearts. | ||
Cell Rep COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation. | ||
J Pathol Clinical response to chemotherapy in oesophageal adenocarcinoma patients is linked to defects in mitochondria.
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