CoraLite®488-conjugated COXIV Monoclonal antibody

COXIV Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra), IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

3C7D2

Cat No : CL488-60251

Print datasheet

Synonyms

COX IV 1, COX4, COX4I1, COXIV



经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

发表文章中的应用

IFSee 1 publications below

产品信息

The immunogen of CL488-60251 is COXIV Fusion Protein expressed in E. coli.

Tested Applications FC (Intra), IF
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COXIV fusion protein Ag20551 种属同源性预测
Full Name cytochrome c oxidase subunit IV isoform 1
Synonyms COX IV 1, COX4, COX4I1, COXIV
Calculated molecular weight19.6 kDa
Observed molecular weight 17-18 kDa
GenBank accession numberBC021236
Gene symbol COX4I1
UniprotP13073
Gene ID (NCBI) 1327
RRIDAB_2883126
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.

实验方案

Product Specific Protocols
IF protocol for CL488 COXIV antibody CL488-60251Download protocol
FC protocol for CL488 COXIV antibody CL488-60251Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Life Sci

Short-term but not long-term high fat diet feeding protects against pressure overload-induced heart failure through activation of mitophagy.

Authors - Yanzhen Tan