验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, mouse testis tissue, RAW264.7, MCF-7 cells, Ramos cells, NIH/3T3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human placenta tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CSE1L Ready-To-Use IHC Kit. CSE1L primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22219-1-AP targets CSE1L in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | CSE1L fusion protein Ag17556 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CSE1 chromosome segregation 1-like (yeast) |
| 别名 | CAS, CSE1, CSE1L, Exp2, Exportin 2, Importin alpha re exporter, XPO2 |
| 计算分子量 | 971 aa, 110 kDa |
| 观测分子量 | 100-110 kDa |
| GenBank蛋白编号 | BC109313 |
| 基因名称 | CSE1L |
| Gene ID (NCBI) | 1434 |
| RRID | AB_10950892 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55060 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSE1L (Chromosome Segregation 1-Like) is located on 20q13.13, and encodes a 971-amino-acid protein. It associates with microtubules and mitotic spindles, and is also considered to have a role in tumor progression. The CSE1L protein includes a nuclear localization signal allowing for transport into the nucleus via the importin-alpha/beta heterodimer. In addition, CSE1L plays roles in cell proliferation and apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CSE1L antibody 22219-1-AP | Download protocol |
| IHC protocol for CSE1L antibody 22219-1-AP | Download protocol |
| IP protocol for CSE1L antibody 22219-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk | ||
Cancers (Basel) GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells. | ||
Sci Rep CSE1L interaction with MSH6 promotes osteosarcoma progression and predicts poor patient survival.
| ||
J Biol Chem RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome. | ||
Int J Oncol Butyrate supplementation regulates expression of chromosome segregation 1‑like protein to reverse the genetic distortion caused by p53 mutations in colorectal cancer.
| ||
J Cancer CSE1L, as a novel prognostic marker, promotes pancreatic cancer proliferation by regulating the AKT/mTOR signaling pathway.
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