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  • Featured Product
  • KD/KO Validated

CXCR4 Monoclonal antibody, PBS Only

CXCR4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4B5E4

Cat no : 60042-1-PBS

Print datasheet

Synonyms

CXCR-4, CXC-R4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4

验证数据展示

  • WB analysis using 60042-1-Ig (same clone as 60042-1-PBS)

    WB analysis using 60042-1-Ig (same clone as 60042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

    IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CXCR4 antibody included
  • IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

    IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CXCR4 antibody included
Planning an IHC experiment? We recommend our IHCeasy CXCR4 Ready-To-Use IHC Kit. CXCR4 primary antibody included.

产品信息

60042-1-PBS targets CXCR4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen CXCR4 fusion protein Ag1528 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name chemokine (C-X-C motif) receptor 4
Synonyms CXCR-4, CXC-R4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4
Calculated Molecular Weight 352 aa, 40 kDa
Observed Molecular Weight 60~70 kDa
GenBank Accession NumberBC020968
Gene Symbol CXCR4
Gene ID (NCBI) 7852
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP61073
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).