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验证数据展示

  • WB analysis using 60042-1-Ig (same clone as 60042-1-PBS)

    WB analysis using 60042-1-Ig (same clone as 60042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

    IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CXCR4 antibody included
  • IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

    IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CXCR4 antibody included
  • IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

    IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CXCR4 antibody included
  • Featured Product
  • KD/KO验证

CXCR4 Monoclonal antibody, PBS Only

CXCR4 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60042-1-PBS

产品说明书

CloneNo. 4B5E4

宿主/亚型

Mouse / IgM

种属反应性

human

应用

WB, IHC, ELISA

别名

CXCR-4, CXC-R4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CXCR4 Ready-To-Use IHC Kit. CXCR4 primary antibody included.

产品信息

60042-1-PBS targets CXCR4 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 CXCR4 fusion protein Ag1528 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 chemokine (C-X-C motif) receptor 4
别名 CXCR-4, CXC-R4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4
计算分子量 352 aa, 40 kDa
观测分子量 60~70 kDa
GenBank蛋白编号BC020968
基因名称 CXCR4
Gene ID (NCBI) 7852
RRIDAB_2091809
偶联类型 Unconjugated
形式 Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDP61073
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).