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  • Featured Product
  • KD/KO Validated

Calnexin Monoclonal antibody, PBS Only

Calnexin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A2C6

Cat no : 66903-1-PBS

Print datasheet

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90

验证数据展示

  • IF Staining of HeLa using 66903-1-Ig (same clone as 66903-1-PBS)

    IF Staining of HeLa using 66903-1-Ig (same clone as 66903-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66903-1-Ig (Calnexin antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). Red: staning with CoraLite555-Phalloidin. This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

  • WB analysis using 66903-1-Ig (same clone as 66903-1-PBS)

    WB analysis using 66903-1-Ig (same clone as 66903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66903-1-Ig (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66903-1-Ig (same clone as 66903-1-PBS)

    WB analysis of HEK-293 using 66903-1-Ig (same clone as 66903-1-PBS)

    WB result of Calnexin antibody (66903-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calnexin transfected HEK-293 cells. This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66903-1-Ig (same clone as 66903-1-PBS)

    WB analysis of HEK-293 using 66903-1-Ig (same clone as 66903-1-PBS)

    HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 66903-1-Ig (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

  • IHC staining of human hepatocirrhosis using 66903-1-Ig (same clone as 66903-1-PBS)

    IHC staining of human hepatocirrhosis using 66903-1-Ig (same clone as 66903-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66903-1-Ig (Calnexin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calnexin antibody included
Planning an IHC experiment? We recommend our IHCeasy Calnexin Ready-To-Use IHC Kit. Calnexin primary antibody included.

产品信息

66903-1-PBS targets Calnexin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Calnexin fusion protein Ag25535 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calnexin
Synonyms calnexin, CANX, CNX, FLJ26570, IP90, P90
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC003552
Gene Symbol Calnexin
Gene ID (NCBI) 821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27824
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.