验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
26758-1-AP targets Cas9 in WB, IP, ELISA applications and shows reactivity with bacteria samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | bacteria |
| 文献引用反应性 | human |
| 免疫原 | Cas9 fusion protein Ag25281 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Cas9 |
| 别名 | Cas9 7A9, CRISPR, CRISPR/Cas9, CRISPR-associated endonuclease Cas9, csn1 |
| 计算分子量 | 160 kDa |
| GenBank蛋白编号 | NP_269215 |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2918109 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99ZW2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes. This antibody detects the sp Cas9.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cas9 antibody 26758-1-AP | Download protocol |
| IP protocol for Cas9 antibody 26758-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Epigenetics E2F1 combined with LINC01004 super-enhancer to promote hepatocellular carcinoma cell proliferation and metastasis |