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验证数据展示

  • WB analysis of HEK-293 using 24777-1-AP

    WB analysis of HEK-293 using 24777-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24777-1-AP (Caspase 2/p18 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 24777-1-AP

    IHC staining of human liver cancer using 24777-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caspase 2 antibody included
  • IHC staining of human liver cancer using 24777-1-AP

    IHC staining of human liver cancer using 24777-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caspase 2 antibody included
  • IF Staining of HEK-293 using 24777-1-AP

    IF Staining of HEK-293 using 24777-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24777-1-AP (Caspase 2/P18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Caspase 2/P18 Polyclonal antibody

Caspase 2/P18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24777-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CASP2, Caspase 2, CASP 2, CASP-2, Caspase 2/p32/p18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy Caspase 2 Ready-To-Use IHC Kit. Caspase 2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24777-1-AP targets Caspase 2/P18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Caspase 2/P18 fusion protein Ag20141 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caspase 2, apoptosis-related cysteine peptidase
别名 CASP2, Caspase 2, CASP 2, CASP-2, Caspase 2/p32/p18
计算分子量 452 aa, 51 kDa
观测分子量48-51 kDa, 32 kDa
GenBank蛋白编号BC002427
基因名称 Caspase 2
Gene ID (NCBI) 835
RRIDAB_2879715
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42575
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).

实验方案

Product Specific Protocols
WB protocol for Caspase 2/P18 antibody 24777-1-APDownload protocol
IHC protocol for Caspase 2/P18 antibody 24777-1-APDownload protocol
IF protocol for Caspase 2/P18 antibody 24777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols