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Caveolin-1 Recombinant antibody

Caveolin-1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240985E4

Cat No : 84044-1-RR

Print datasheet

Synonyms

Caveolin 1, CAV1, CGL3, CAV, BSCL3

验证数据展示

  • WB analysis using 84044-1-RR

    WB analysis using 84044-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84044-1-RR (Caveolin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84044-1-RR

    Affinity and Kinetic Characterization of 84044-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84044-1-RR against Human Caveolin-1 were performed. The affinity constant is 1.53 nM.

经过测试的应用

Positive WB detected inNIH/3T3 cells, A549 cells, MDA-MB-231 cells
Planning an IHC experiment? We recommend our IHCeasy Caveolin-1 Ready-To-Use IHC Kit. Caveolin-1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84044-1-RR is Caveolin-1 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Caveolin-1 fusion protein Ag8049 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name caveolin 1, caveolae protein, 22kDa
Synonyms Caveolin 1, CAV1, CGL3, CAV, BSCL3
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC006432
Gene Symbol CAV1
Gene ID (NCBI) 857
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ03135
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).

实验方案

Product Specific Protocols
WB protocol for Caveolin-1 antibody 84044-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols