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验证数据展示

  • WB analysis using 84044-1-RR

    WB analysis using 84044-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84044-1-RR (Caveolin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 84044-1-RR

    IHC staining of human colon using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human colon cancer using 84044-1-RR

    IHC staining of human colon cancer using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human lung squamous cell carcinoma using 84044-1-RR

    IHC staining of human lung squamous cell carcinoma using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • Affinity and Kinetic Characterization of 84044-1-RR

    Affinity and Kinetic Characterization of 84044-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84044-1-RR against Human Caveolin-1 were performed. The affinity constant is 1.53 nM.

Caveolin-1 Recombinant monoclonal antibody

Caveolin-1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84044-1-RR

产品说明书

CloneNo. 240985E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Caveolin 1, CAV1, CGL3, CAV, BSCL3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, A549 cells, MDA-MB-231 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue, human lung squamous cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Caveolin-1 Ready-To-Use IHC Kit. Caveolin-1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84044-1-RR targets Caveolin-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8049

Product name: Recombinant human Caveolin-1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 11-179 aa of BC006432

Sequence: GHLYTVPIREQGNIYKPNNKAMADELSEKQVYDAHTKEIDLVNRDPKHLNDDVVKIDFEDVIAEPEGTHSFDGIWKASFTTFTVTKYWFYRLLSALFGIPMALIWGIYFAILSFLHIWAVVPCIKSFLIEIQCISRVYSIYVHTVCDPLFEAVGKIFSNVRINLQKEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 caveolin 1, caveolae protein, 22kDa
别名 Caveolin 1, CAV1, CGL3, CAV, BSCL3
计算分子量 22 kDa
观测分子量 20-25 kDa
GenBank蛋白编号BC006432
基因名称 CAV1
Gene ID (NCBI) 857
RRIDAB_3671611
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ03135
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).

实验方案

Product Specific Protocols
WB protocol for Caveolin-1 antibody 84044-1-RRDownload protocol
IHC protocol for Caveolin-1 antibody 84044-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols