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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 60277-1-Ig (same clone as 60277-1-PBS)

    WB analysis using 60277-1-Ig (same clone as 60277-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60277-1-Ig (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60277-1-Ig (same clone as 60277-1-PBS)

    IHC staining of human breast cancer using 60277-1-Ig (same clone as 60277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60277-1-Ig (Chk1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHK1 antibody included

Chk1 Monoclonal antibody, PBS Only

Chk1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60277-1-PBS

产品说明书

CloneNo. 3A3A5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

CHEK1, Chk1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.

产品信息

60277-1-PBS targets Chk1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Chk1 fusion protein Ag0409 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CHK1 checkpoint homolog (S. pombe)
别名 CHEK1, Chk1
计算分子量 54 kDa
观测分子量50 kDa
GenBank蛋白编号BC004202
基因名称 Chk1
Gene ID (NCBI) 1111
RRIDAB_2881396
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14757
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.