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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 60277-1-Ig (same clone as 60277-1-PBS)

    WB analysis using 60277-1-Ig (same clone as 60277-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60277-1-Ig (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60277-1-Ig (same clone as 60277-1-PBS)

    IHC staining of human breast cancer using 60277-1-Ig (same clone as 60277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60277-1-Ig (Chk1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHK1 antibody included

Chk1 Monoclonal antibody, PBS Only

Chk1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60277-1-PBS

产品说明书

CloneNo. 3A3A5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

CHEK1, Chk1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.

产品信息

60277-1-PBS targets Chk1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0409

Product name: Recombinant human CHK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 42-256 aa of BC004202

Sequence: MKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CHK1 checkpoint homolog (S. pombe)
别名 CHEK1, Chk1
计算分子量 54 kDa
观测分子量50 kDa
GenBank蛋白编号BC004202
基因名称 Chk1
Gene ID (NCBI) 1111
RRIDAB_2881396
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14757
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.