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  • Featured Product
  • KD/KO Validated

Cofilin Monoclonal antibody, PBS Only

Cofilin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G6A2

Cat no : 66057-1-PBS

Print datasheet

Synonyms

18 kDa phosphoprotein, CFL, CFL1, Cofilin, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18

验证数据展示

  • WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

    WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66057-1-Ig (same clone as 66057-1-PBS)

    IHC staining of human testis using 66057-1-Ig (same clone as 66057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CFL1 antibody included
  • IHC staining of human testis using 66057-1-Ig (same clone as 66057-1-PBS)

    IHC staining of human testis using 66057-1-Ig (same clone as 66057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CFL1 antibody included
  • IF Staining of HeLa using 66057-1-Ig (same clone as 66057-1-PBS)

    IF Staining of HeLa using 66057-1-Ig (same clone as 66057-1-PBS)

    Immunofluorescent analysis of Hela cells using 66057-1-Ig (Cofilin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

    WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CFL1 Ready-To-Use IHC Kit. CFL1 primary antibody included.

产品信息

66057-1-PBS targets Cofilin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen Cofilin fusion protein Ag18044 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name cofilin 1 (non-muscle)
Synonyms 18 kDa phosphoprotein, CFL, CFL1, Cofilin, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC012318
Gene Symbol Cofilin
Gene ID (NCBI) 1072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23528
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.