CoraLite647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L)

Applications

IF, FC

Conjugate

CoraLite®647

Host

Donkey

Reactivity

Mouse

Cat No : SA00014-8

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66009-1-Ig (Beta Actin antiboody) and CoraLite®647 conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) antibody (SA00014-8) at dilution of 1:150.
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-human RBAP48 Monoclonal antibody (66060-1-Ig) and CoraLite®647-Conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications	IF, FC
Host Species	Donkey
Species Reactivity	Mouse
Physical State	Liquid
Suggested Dilution Range	1:100 – 1:500
Purity	The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Storage Buffer	0.01 M Sodium phosphate, 0.25 M NaCl, 50% glycerol, 5 mg/ml BSA, 0.01% Sodium Azide, pH 7.6.
Preservative	0.01% Sodium Azide
Storage Instructions	Store at -20°C. Stable for one year after shipment.
Fluorophore	CL647 Fluorescent Dye. Amax=642nm, Emax=662nm
Stabilizer	5 mg/ml BSA
Absorbance Ratio	A651/A280 = 4.0
Clonality	Polyclonal

Title
Liver Int Biglycan promotes hepatic fibrosis through activating heat shock protein 47 Authors - Mengli Yu View Article

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