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验证数据展示

  • WB analysis of human brain using 55182-1-AP

    WB analysis of human brain using 55182-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55182-1-AP (DCTN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 55182-1-AP

    IHC staining of human brain using 55182-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with DCTN1 antibody included
  • IHC staining of human brain using 55182-1-AP

    IHC staining of human brain using 55182-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with DCTN1 antibody included
  • WB analysis of HeLa using 55182-1-AP

    WB analysis of HeLa using 55182-1-AP

    WB result of DCTN1/p150 glued antibody (55182-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCTN1/p150 glued transfected HeLa cells.

  • IF Staining of MDCK using 55182-1-AP

    IF Staining of MDCK using 55182-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 55182-1-AP (p150 glued antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

DCTN1/p150 glued Polyclonal antibody

DCTN1/p150 glued Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55182-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, canine and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Synonyms

DCTN1, p150 glued, DP-150, DCTN 1, DAP-150

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, HeLa cells, Jurkat cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells
Planning an IHC experiment? We recommend our IHCeasy DCTN1 Ready-To-Use IHC Kit. DCTN1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55182-1-AP targets DCTN1/p150 glued in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, canine
Cited Reactivityhuman, mussel
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynactin 1 (p150, glued homolog, Drosophila)
Synonyms DCTN1, p150 glued, DP-150, DCTN 1, DAP-150
Calculated Molecular Weight 142 kDa
Observed Molecular Weight 135 kDa, 150 kDa
GenBank Accession NumberNM_004082
Gene Symbol p150 glued
Gene ID (NCBI) 1639
RRIDAB_10837074
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14203
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCTN1, also named as DAP-150, p150-glued and p135, belongs to the dynactin 150 kDa subunit family. It is required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B). This antibody is specific to DCTN1.

实验方案

Product Specific Protocols
WB protocol for DCTN1/p150 glued antibody 55182-1-APDownload protocol
IHC protocol for DCTN1/p150 glued antibody 55182-1-APDownload protocol
IF protocol for DCTN1/p150 glued antibody 55182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
musselWB

Ecotoxicol Environ Saf

Proteomic profile in the mussel Perna viridis after short-term exposure to the brown tide alga Aureococcus anophagefferens.

Authors - Jian-Wei Zheng
View Article
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
View Article