验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, HeLa cells, Jurkat cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
Planning an IHC experiment? We recommend our IHCeasy DCTN1 Ready-To-Use IHC Kit. DCTN1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
55182-1-AP targets DCTN1/p150 glued in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, canine |
| 文献引用反应性 | human, mussel |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dynactin 1 (p150, glued homolog, Drosophila) |
| 别名 | DCTN1, p150 glued, DP-150, DCTN 1, DAP-150 |
| 计算分子量 | 142 kDa |
| 观测分子量 | 135 kDa, 150 kDa |
| GenBank蛋白编号 | NM_004082 |
| 基因名称 | p150 glued |
| Gene ID (NCBI) | 1639 |
| RRID | AB_10837074 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14203 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCTN1, also named as DAP-150, p150-glued and p135, belongs to the dynactin 150 kDa subunit family. It is required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B). This antibody is specific to DCTN1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DCTN1/p150 glued antibody 55182-1-AP | Download protocol |
| IHC protocol for DCTN1/p150 glued antibody 55182-1-AP | Download protocol |
| IF protocol for DCTN1/p150 glued antibody 55182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf Proteomic profile in the mussel Perna viridis after short-term exposure to the brown tide alga Aureococcus anophagefferens. | ||
