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  • Featured Product
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DEF6 Polyclonal antibody

DEF6 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11369-1-AP

Print datasheet

Synonyms

SLAT, IRF4-binding protein, IRF4 binding protein, IBP, DEF-6

验证数据展示

  • WB analysis of Jurkat using 11369-1-AP

    WB analysis of Jurkat using 11369-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11369-1-AP (DEF6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 11369-1-AP

    WB analysis of Jurkat using 11369-1-AP

    WB result of DEF6 antibody (11369-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEF6 transfected Jurkat cells.

  • IHC staining of mouse spleen using 11369-1-AP

    IHC staining of mouse spleen using 11369-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11369-1-AP (DEF6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DEF6 antibody included
  • IHC staining of mouse spleen using 11369-1-AP

    IHC staining of mouse spleen using 11369-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11369-1-AP (DEF6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DEF6 antibody included
  • IP experiment of Jurkat using 11369-1-AP

    IP experiment of Jurkat using 11369-1-AP

    IP result of anti-DEF6 (IP:11369-1-AP, 4ug; Detection:11369-1-AP 1:600) with Jurkat cells lysate 2400ug.

  • IHC staining of human tonsillitis using 11369-1-AP

    IHC staining of human tonsillitis using 11369-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11369-1-AP (DEF6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DEF6 antibody included

经过测试的应用

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inmouse spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DEF6 Ready-To-Use IHC Kit. DEF6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11369-1-AP targets DEF6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen DEF6 fusion protein Ag1925 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name differentially expressed in FDCP 6 homolog (mouse)
Synonyms SLAT, IRF4-binding protein, IRF4 binding protein, IBP, DEF-6
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC017504
Gene Symbol DEF6
Gene ID (NCBI) 50619
RRIDAB_2230504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4E7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DEF6 antibody 11369-1-APDownload protocol
IHC protocol for DEF6 antibody 11369-1-APDownload protocol
IP protocol for DEF6 antibody 11369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols