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验证数据展示

  • IHC staining of mouse cerebellum using 24923-1-AP

    IHC staining of mouse cerebellum using 24923-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24923-1-AP (DHX35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DHX35 Polyclonal antibody

DHX35 Polyclonal Antibody for IHC, ELISA
Cat No. 24923-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

C20orf15, DDX35, DEAH box protein 35, DHX35, KAIA0875

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24923-1-AP targets DHX35 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen DHX35 fusion protein Ag20693 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 35
Synonyms C20orf15, DDX35, DEAH box protein 35, DHX35, KAIA0875
Calculated Molecular Weight 703 aa, 79 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC132669
Gene Symbol DHX35
Gene ID (NCBI) 60625
RRIDAB_2879798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H5Z1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for DHX35 antibody 24923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols