验证数据展示

IHC staining of human breast cancer using 11355-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11355-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP experiment of mouse liver using 11355-1-AP
IP result of anti-DHX58/LGP2 (IP:11355-1-AP, 3ug; Detection:11355-1-AP 1:300) with mouse liver tissue lysate 6000ug.
WB analysis of HEK-293 using 11355-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11355-1-AP (DHX58/LGP2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 11355-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human nephroblastoma using 11355-1-AP
Immunohistochemical analysis of paraffin-embedded human nephroblastoma tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human nephroblastoma using 11355-1-AP
Immunohistochemical analysis of paraffin-embedded human nephroblastoma tissue slide using 11355-1-AP (DHX58/LGP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO验证

DHX58/LGP2 Polyclonal antibody

DHX58/LGP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11355-1-AP

产品说明书

引用文献(16)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IP, IHC, ELISA and More (3)

别名

D11LGP2, D11lgp2e, DHX58, DHX58/LGP2, LGP2, Protein D11Lgp2 homolog, RIG I like receptor LGP2, RLR

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格:

-/ -

点击咨询售前客服

点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (16)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, rat kidney tissue, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human breast cancer tissue, human nephroblastoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

应用	推荐稀释比
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 4 publications below
WB	See 15 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 2 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

产品信息

11355-1-AP targets DHX58/LGP2 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IP, IHC, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, RIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, pig, monkey, chicken
免疫原	DHX58/LGP2 fusion protein Ag1910 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	DEXH (Asp-Glu-X-His) box polypeptide 58
别名	D11LGP2, D11lgp2e, DHX58, DHX58/LGP2, LGP2, Protein D11Lgp2 homolog, RIG I like receptor LGP2, RLR
计算分子量	678 aa, 77 kDa
观测分子量	77 kDa
GenBank蛋白编号	BC014949
基因名称	DHX58
Gene ID (NCBI)	79132
RRID	AB_2092319
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q96C10
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

DHX58(Probable ATP-dependent RNA helicase DHX58) is also named as D11LGP2E, LGP2 and belongs to the RLR subfamily. DHX58, originally identified as a highly expressed gene in mammary tumors, is another cytoplasmic DEX(D/H)-box helicase that can recognize RNA(PMID: 18411269). It acts as a positive, but not negative, regulator of RIG-I-and MDA5-dependent recognition of RNA virus infection and plays a pivotal role in antiviral responses in vivo(PMID:20080593).

实验方案

Product Specific Protocols
WB protocol for DHX58/LGP2 antibody 11355-1-AP	Download protocol
IHC protocol for DHX58/LGP2 antibody 11355-1-AP	Download protocol
IP protocol for DHX58/LGP2 antibody 11355-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Nat Commun RIG-I-like receptors direct inflammatory macrophage polarization against West Nile virus infection. Authors - Amy E L Stone KO Validated View Article
human	WB	Nucleic Acids Res miR-122 does not impact recognition of the HCV genome by innate sensors of RNA but rather protects the 5' end from the cellular pyrophosphatases, DOM3Z and DUSP11. Authors - Yalena Amador-Cañizares View Article
human	WB	EMBO Rep RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling. Authors - Jean-Patrick Parisien View Article
human,pig,monkey	WB,IF,IP	J Virol LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling Authors - Zhenbang Zhu KD Validated View Article
human,mouse	WB	J Virol Ambivalent role of the innate immune response in rabies virus pathogenesis. Authors - Chopy Damien D View Article
human,mouse	WB,IHC,IP	Cancer Sci Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma. Authors - Li-Ling Lin View Article

验证数据展示

IHC staining of human breast cancer using 11355-1-AP

IHC staining of human breast cancer using 11355-1-AP

IP experiment of mouse liver using 11355-1-AP

WB analysis of HEK-293 using 11355-1-AP

WB analysis using 11355-1-AP

IHC staining of human nephroblastoma using 11355-1-AP

IHC staining of human nephroblastoma using 11355-1-AP

DHX58/LGP2 Polyclonal antibody

DHX58/LGP2 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

RIG-I-like receptors direct inflammatory macrophage polarization against West Nile virus infection.

miR-122 does not impact recognition of the HCV genome by innate sensors of RNA but rather protects the 5' end from the cellular pyrophosphatases, DOM3Z and DUSP11.

RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling.

LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling

Ambivalent role of the innate immune response in rabies virus pathogenesis.

Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma.