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DKK1 Polyclonal antibody

DKK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21112-1-AP

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Synonyms

Dickkopf 1, Dickkopf related protein 1, DKK 1, DKK1, hDkk 1, SK



经过测试的应用

Positive WB detected inA549 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman gliomas tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21112-1-AP is DKK1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit
Immunogen DKK1 fusion protein Ag15110 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dickkopf homolog 1 (Xenopus laevis)
Synonyms Dickkopf 1, Dickkopf related protein 1, DKK 1, DKK1, hDkk 1, SK
Calculated Molecular Weight 266 aa, 29 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC001539
Gene Symbol DKK1
Gene ID (NCBI) 22943
RRIDAB_10733097
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94907
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kDa doublet protein, which is larger than the deduced molecular mass of 26 kDa. Sometime DKK1 is expressed as a 42- to 50-kDa secreted protein, with little change observed after glycanase treatment.

实验方案

Product Specific Protocols
WB protocol for DKK1 antibody 21112-1-APDownload protocol
IHC protocol for DKK1 antibody 21112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Nat Commun

Cancer stem cell regulated phenotypic plasticity protects metastasized cancer cells from ferroptosis.

Authors - Mingming Wu
  • KD Validated
humanWB

Bioengineered

Exosomes from bone marrow mesenchymal stem cells promoted osteogenic differentiation by delivering miR-196a that targeted Dickkopf-1 to activate Wnt/β-catenin pathway

Authors - Zhi Peng
humanWB

Oncotarget

MicroRNA-372 inhibits endometrial carcinoma development by targeting the expression of the Ras homolog gene family member C (RhoC).

Authors - Bo-Liang Liu
ratWB

Cells

Sirtuin 6 Overexpression Improves Rotator Cuff Tendon-to-Bone Healing in the Aged

Authors - Young Jae Moon
ratWB

Osteoporos Int

Rehmanniae Radix Preparata suppresses bone loss and increases bone strength through interfering with canonical Wnt/β-catenin signaling pathway in OVX rats.

Authors - C Liu
humanWB

Biochem Pharmacol

miR-29a-3p-dependent COL3A1 and COL5A1 expression reduction assists sulforaphane to inhibit gastric cancer progression.

Authors - Sichong Han