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DNMT3A Polyclonal antibody

DNMT3A Polyclonal Antibody for IHC, ELISA
Cat No. 19366-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

IHC, ELISA and More (2)

DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, DNMT3A, DNMT3A2, M.HsaIIIA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman kidney tissue, human lung cancer tissue, mouse heart tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DNMT3A Ready-To-Use IHC Kit. DNMT3A primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19366-1-AP targets DNMT3A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, pig
免疫原 DNMT3A fusion protein Ag7015 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DNA (cytosine-5-)-methyltransferase 3 alpha
别名 DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, DNMT3A, DNMT3A2, M.HsaIIIA
计算分子量 912 aa, 102 kDa
GenBank蛋白编号BC043617
基因名称 DNMT3A
Gene ID (NCBI) 1788
RRIDAB_10642139
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6K1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.

实验方案

Product Specific Protocols
IHC protocol for DNMT3A antibody 19366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nucleic Acids Res

YY1 safeguard multidimensional epigenetic landscape associated with extended pluripotency.

Authors - Xiaotao Dong
humanIHC,IF

Commun Biol

EZH2 elicits CD8+ T-cell desert in esophageal squamous cell carcinoma via suppressing CXCL9 and dendritic cells

Authors - Chun-Yan Zhu
mouseWB

Development

RNA ligase RTCB regulates mRNA Alternative splicing and is required for mouse oocyte development and maintenance

Authors - Hua Zhang
humanWB

Aging (Albany NY)

ACADS acts as a potential methylation biomarker associated with the proliferation and metastasis of hepatocellular carcinomas.

Authors - Diyu Chen
humanWB

Biochim Biophys Acta

HIC1 epigenetically represses CIITA transcription in B lymphocytes.

Authors - Sheng Zeng
  • KD Validated
humanWB

J Appl Toxicol

Protein profiles of cardiomyocyte differentiation in murine embryonic stem cells exposed to perfluorooctane sulfonate.

Authors - Ying-Ying Zhang
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