• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DTX3L Polyclonal antibody

DTX3L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11963-1-AP

Print datasheet

Synonyms

Protein deltex 3 like, EC:2.3.2.27, E3 ubiquitin-protein ligase DTX3L, deltex 3 like (Drosophila), B-lymphoma- and BAL-associated protein

验证数据展示

  • WB analysis of MCF-7 using 11963-1-AP

    WB analysis of MCF-7 using 11963-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11963-1-AP (DTX3L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 11963-1-AP

    IHC staining of human ovary cancer using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11963-1-AP targets DTX3L in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, goat
Immunogen DTX3L fusion protein Ag2568 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deltex 3-like (Drosophila)
Synonyms Protein deltex 3 like, EC:2.3.2.27, E3 ubiquitin-protein ligase DTX3L, deltex 3 like (Drosophila), B-lymphoma- and BAL-associated protein
Calculated Molecular Weight 740 aa, 84 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC060509
Gene Symbol DTX3L
Gene ID (NCBI) 151636
RRIDAB_874119
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDB6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DTX3L antibody 11963-1-APDownload protocol
IHC protocol for DTX3L antibody 11963-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Sci

Ubiquitination of NOTCH2 by DTX3 suppresses the proliferation and migration of human esophageal carcinoma.

Authors - Xin-Yu Ding
View Article
goatIHC

Biomolecules

Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats.

Authors - Xupeng Zang
View Article
humanWB

Cancer Cell Int

Circular RNA circEYA3 promotes the radiation resistance of hepatocellular carcinoma via the IGF2BP2/DTX3L axis

Authors - Pan Hu
View Article