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  • Featured Product
  • KD/KO Validated

DUS3L Polyclonal antibody

DUS3L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15643-1-AP

Print datasheet

Synonyms

DUS3, DUS3L

验证数据展示

  • WB analysis using 15643-1-AP

    WB analysis using 15643-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15643-1-AP (DUS3L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 15643-1-AP

    IHC staining of human breast cancer using 15643-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15643-1-AP (DUS3L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 15643-1-AP

    IHC staining of human breast cancer using 15643-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15643-1-AP (DUS3L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15643-1-AP targets DUS3L in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DUS3L fusion protein Ag8142 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dihydrouridine synthase 3-like (S. cerevisiae)
Synonyms DUS3, DUS3L
Calculated Molecular Weight 46 kDa, 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC004549
Gene Symbol DUS3L
Gene ID (NCBI) 56931
RRIDAB_2293096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96G46
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DUS3L antibody 15643-1-APDownload protocol
IHC protocol for DUS3L antibody 15643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Chem Biol

Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.

Authors - Wei Dai
  • KD Validated
View Article