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验证数据展示

  • WB analysis using 10739-1-AP

    WB analysis using 10739-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10739-1-AP (MKP-2/DUSP4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of MDA-MB-231 using 10739-1-AP

    IP experiment of MDA-MB-231 using 10739-1-AP

    IP result of anti-MKP-2/DUSP4 (IP:10739-1-AP, 4ug; Detection:10739-1-AP 1:2000) with MDA-MB-231 cells lysate 1280 ug.

  • FC experiment of MDA-MB-231 using 10739-1-AP

    FC experiment of MDA-MB-231 using 10739-1-AP

    1x10^6 MDA-MB-231 cells were intracellularly stained with 0.25 ug MKP-2/DUSP4 Polyclonal antibody (10739-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

MKP-2/DUSP4 Polyclonal antibody

MKP-2/DUSP4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 10739-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA and More (1)

别名

DUSP4, MKP-2, dual specificity phosphatase 4, EC:3.1.3.16, EC:3.1.3.48

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHCT 116 cells, SK-BR-3 cells
Positive IP detected inMDA-MB-231 cells
Positive FC (Intra) detected inMDA-MB-231 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10739-1-AP targets MKP-2/DUSP4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 MKP-2/DUSP4 fusion protein Ag1206 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dual specificity phosphatase 4
别名 DUSP4, MKP-2, dual specificity phosphatase 4, EC:3.1.3.16, EC:3.1.3.48
计算分子量 43 kDa
观测分子量 40-43 kDa
GenBank蛋白编号BC014565
基因名称 DUSP4
Gene ID (NCBI) 1846
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13115
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus.

实验方案

Product Specific Protocols
WB protocol for MKP-2/DUSP4 antibody 10739-1-APDownload protocol
IP protocol for MKP-2/DUSP4 antibody 10739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

J Clin Invest

Compound haploinsufficiency of Dok2 and Dusp4 promotes lung tumorigenesis.

Authors - Ming Chen
  • KO Validated
View Article
mouseWB

Cell Death Discov

NR4A1 enhances MKP7 expression to diminish JNK activation induced by ROS or ER-stress in pancreatic β cells for surviving.

Authors - Ze-Qing Pu
View Article