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ChromoTek DYKDDDDK Fab-Trap® Agarose Kit

The ChromoTek DYKDDDDK Fab-Trap® Agarose Kit consists of an anti-DYKDDDDK-tag Fab fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap® Agarose Kit is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc

Specificity

Flag®-tag (1x and 3x)

Applications

IP, CoIP, ChIP, RIP, Protein purification

Type

Fab-fragment

Conjugate

Agarose

Cat No : ffak

Synonyms

Fab-Trap Agarose, Fab-Trap, DYKDDDDK, flag

Validation Data Gallery

  • DYKDDDDK Fab-Trap® was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

    DYKDDDDK Fab-Trap® was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

  • DYKDDDDK Fab-Trap® was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 2x SDS-sample buffer the enriched Flag® fusion protein and Fab-fragment are released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

    DYKDDDDK Fab-Trap® was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 2x SDS-sample buffer the enriched Flag® fusion protein and Fab-fragment are released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

  • DYKDDDDK Fab-Trap® was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

    DYKDDDDK Fab-Trap® was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

Product Information

The ChromoTek DYKDDDDK Fab-Trap® Agarose Kit consists of an anti-DYKDDDDK-tag Fab fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap® Agarose Kit is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc

DescriptionThe DYKDDDDK Fab-Trap Agarose Kit contains DYKDDDDK Fab-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of Flag®-tagged proteins and their interacting factors.

• Lowest background in class

• Less contaminating antibody chains

• Recombinantly expressed Fab-fragment derived

• Compatible with harsh buffer conditions

• Ready-to-use

ApplicationsIP, CoIP, ChIP, RIP, Protein purification
Specificity/TargetThe DYKDDDDK Fab-Trap® Agarose specifically binds to 1x and 3xDYKDDDDK-tag sequence (1x and 3xFlag®-tag). It binds to same epitope as Sigma's Anti-FLAG® Antibody and is compatible with N-, C-terminal or internal tagging.
Binding capacity12.5 - 15 µg of recombinant 3xDYKDDDDK-tagged protein (~30 kDa) per 25µL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution buffer3xDYKDDDDK-peptide
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility0.5 % Nonidet P40 Substitute, 5 % Tween™ 20, 2 M NaCl, 10 mM TCEP, 10 mM DTT, 10 mM ß-mercaptoethanol, 2 M Urea
Type Fab-fragment
ClassRecombinant
HostMouse
RRIDAB_2894836
Storage Buffer1x PBS, Preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

Component Description
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash bufferRemoval of unwanted proteins, peptides, etc.
DYKDDDDK Fab-Trap™ Agarose20 reactions (500 µL slurry)

Publications

ApplicationTitle
CoIP

Nat Commun

A spatio-temporally constrained gene regulatory network directed by PBX1/2 acquires limb patterning specificity via HAND2

Authors - Marta Losa
View Article
CoIP

EMBO Mol Med

A missense mutation in human INSC causes peripheral neuropathy

Authors - Jui-Yu Yeh
View Article
WB

EMBO J

Stereoisomer-specific reprogramming of a bacterial flagellin sialyltransferase

Authors - Nicolas Kint
View Article
CoIP

Sci Adv

Nuclear pyruvate dehydrogenase complex regulates histone acetylation and transcriptional regulation in the ethylene response

Authors - Zhengyao Shao
View Article

Biofactors

BAG3 regulates cilia homeostasis of glioblastoma via its WW domain

Authors - Caterina Roth
View Article
CoIP

J Cell Physiol

KDF1 promotes ameloblast differentiation by inhibiting the IKK/IκB/NF-κB axis

Authors - Hangbo Liu
View Article