Validation Data Gallery
![DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.](/products/pictures/Result-ffap.png "DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.")
Product Information
The DYKDDDDK Immunoprecipitation Starter Pack is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.
| Description | The DYKDDDDK Immunoprecipitation Starter Pack is used to immunoprecipitate, elute, and detect 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. |
| Applications | IP |
| Host | Mouse |
| Components | DYKDDDDK Fab-Trap™ Agarose (20 reactions, ffa-20) 3xDYKDDDDK-peptide (1mg, fp-1) DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL, 20543-1-AP-20). |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Specificity/Target | Specifically binds to 1x and 3xDYKDDDDK-tag sequence (1x and 3xFlag®-tag). Compatible with N-, C- terminal or internal tagging |
| Binding capacity | 12.5 - 15 µg of recombinant 3xDYKDDDDK-tagged protein (~30 kDa) per 25µL bead slurry |
| Elution buffer | 3xDYKDDDDK-peptide (fp-1) SDS sample buffer 0.2 M glycine pH 2.5 |
| Storage buffer | 1x PBS, Preservative: 0.09 % sodium azide |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
| Stability | Stable for 1 year upon receipt. |
| Shipment | Shipped at ambient temperature. |
Kit components
| Component | Description |
|---|---|
| ffa-20 | DYKDDDDK Fab-Trap™ Agarose (20 reactions) |
| fp-1 | 3xDYKDDDDK-peptide (1mg) |
| 20543-1-AP | DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL) |