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ChromoTek DYKDDDDK Immunoprecipitation Starter Pack

The DYKDDDDK Immunoprecipitation Starter Pack is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

Specificity

1x and 3x Flag®-tag

Applications

IP

Type

Fab-fragment

Conjugate

Agarose

Cat No : ffap

Synonyms

DYKDDDDK, Flag

Validation Data Gallery

  • DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

    DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

Product Information

The DYKDDDDK Immunoprecipitation Starter Pack is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

DescriptionThe DYKDDDDK Immunoprecipitation Starter Pack is used to immunoprecipitate, elute, and detect 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.
HostMouse
ApplicationsIP
ComponentsDYKDDDDK Fab-Trap™ Agarose (20 reactions, ffa-20)
3xDYKDDDDK-peptide (1mg, fp-1)
DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL, 20543-1-AP-20).
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Specificity/TargetSpecifically binds to 1x and 3xDYKDDDDK-tag sequence (1x and 3xFlag®-tag). Compatible with N-, C- terminal or internal tagging
Binding capacity12.5 - 15 µg of recombinant 3xDYKDDDDK-tagged protein (~30 kDa) per 25µL bead slurry
Elution buffer3xDYKDDDDK-peptide (fp-1)
SDS sample buffer
0.2 M glycine pH 2.5
Storage buffer1x PBS, Preservative: 0.09 % sodium azide
Storage ConditionUpon receipt store at +4°C. Do not freeze!
StabilityStable for 1 year upon receipt.
ShipmentShipped at ambient temperature.

Kit components

Component Description
ffa-20DYKDDDDK Fab-Trap™ Agarose (20 reactions)
fp-13xDYKDDDDK-peptide (1mg)
20543-1-APDYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL)