ChromoTek DYKDDDDK Fab-Trap® Agarose
The ChromoTek DYKDDDDK Fab-Trap® Agarose consists of an anti-DYKDDDDK-tag Fab-fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap® Agarose is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.
Specificity
Flag®-tag (1x and 3x)
Applications
IP, CoIP, ChIP, RIP, Protein purification
Type
Fab-fragment
Conjugate
Agarose
Cat No : ffa
Synonyms
Validation Data Gallery
Product Information
The ChromoTek DYKDDDDK Fab-Trap® Agarose consists of an anti-DYKDDDDK-tag Fab-fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap® Agarose is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.
Description | The DYKDDDDK Fab-Trap Agarose is a premium affinity resin for efficient immunoprecipitation (IP) of Flag®-tagged proteins. The DYKDDDDK Fab-Trap Agarose consists of a Fab-fragment, which is covalently bound to agarose beads. • Lowest background in class • Less contaminating antibody chains • Recombinantly expressed Fab-fragment derived • Compatible with harsh buffer conditions • Ready-to-use |
Applications | IP, CoIP, ChIP, RIP, Protein purification |
Specificity/Target | The DYKDDDDK Fab-Trap® Agarose specifically binds to 1x and 3xDYKDDDDK-tag sequence (1x and 3xFlag®-tag). It binds to same epitope as Sigma's Anti-FLAG® Antibody and is compatible with N-, C-terminal or internal tagging. |
Binding capacity | 12.5 - 15 µg of recombinant 3xDYKDDDDK-tagged protein (~30 kDa) per 25µL bead slurry |
Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
Elution buffer | 3xDYKDDDDK-peptide SDS sample buffer 0.2 M glycine pH 2.5 |
Wash buffer compatibility | 0.5 % Nonidet P40 Substitute, 5 % Tween™ 20, 2 M NaCl, 10 mM TCEP, 10 mM DTT, 10 mM ß-mercaptoethanol, 2 M Urea |
Type | Fab-fragment |
Class | Recombinant |
Host | Mouse |
RRID | AB_2894836 |
Storage Buffer | 1x PBS, Preservative: 0.09 % sodium azide |
Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
Publications
Application | Title |
---|---|
EMBO Mol Med Targeting PI3K/Akt/mTOR signaling in rodent models of PMP22 gene-dosage diseases | |
mBio The Cajal body protein p80-coilin forms a complex with the adenovirus L4-22K protein and facilitates the nuclear export of adenovirus mRNA | |
Open Biol Coiled-coil-mediated dimerization of Atg16 is required for binding to the PROPPIN Atg21 | |
J Biol Chem ZNF692 regulates nucleolar morphology by interacting with NPM1 and modifying its self-assembly properties | |
bioRxiv Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins |