ChromoTek DYKDDDDK Fab-Trap™ Agarose

The ChromoTek DYKDDDDK Fab-Trap™ Agarose consists of an anti-DYKDDDDK-tag Fab-fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap™ Agarose is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

Specificity

1x and 3xFlag®-tag

Applications

IP

Conjugate

Agarose

Cat No : ffa

Synonyms

Fab-Trap Agarose, Fab-Trap, DYKDDDDK, flag



Product Information

The ChromoTek DYKDDDDK Fab-Trap™ Agarose consists of an anti-DYKDDDDK-tag Fab-fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap™ Agarose is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

ApplicationsIP
HostMouse
RRIDAB_2894836
SpecificitySpecifically binds to 1x and 3xDYKDDDDK-tag sequence (1x and 3xFlag®-tag). Compatible with N-, C- terminal or internal tagging.
LigandAnti-DYKDDDDK-tag Fab-fragment
Binding capacity12.5 - 15 µg of recombinant 3xDYKDDDDK-tagged protein (~30 kDa) per 25µL bead slurry
CloneFab-fragment of monoclonal mouse IgG1 M2.1
Bead size90 µm (cross-linked 4 % agarose beads)
Storage buffer1x PBS, Preservative: 0.09 % sodium azide
Elution buffer3xDYKDDDDK-peptide (fp-1)
SDS-sample buffer
0.2 M glycine pH 2.5
Storage conditionsUpon receipt store at +4°C. Do not freeze!
StabilityStable for 1 year upon receipt.
ShipmentShipped at ambient temperature.