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ChromoTek DYKDDDDK Fab-Trap™ Agarose

The ChromoTek DYKDDDDK Fab-Trap™ Agarose consists of an anti-DYKDDDDK-tag Fab-fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap™ Agarose is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

Specificity

Flag®-tag (1x and 3x)

Applications

IP, CoIP, ChIP, RIP, Protein purification

Type

Fab-fragment

Conjugate

Agarose

Cat No : ffa

Synonyms

Fab-Trap Agarose, Fab-Trap, DYKDDDDK, flag

Validation Data Gallery

  • DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

    DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

  • DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 2x SDS-sample buffer the enriched Flag® fusion protein and Fab-fragment are released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

    DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 2x SDS-sample buffer the enriched Flag® fusion protein and Fab-fragment are released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, B: Bound.

  • Analysis of unspecific binding of endogenous proteins to the DYKDDDDK Fab-Trap™ in the absence of Flag® fusion protein. Lysates devoid of Flag® fusion protein from HEK cells (HEK293T), Yeast (Saccharomyces cerevisiae), Insect cells (HighFive), Bacteria (Escherichia coli TG1) , and Plant (Arabidopsis thaliana) were subjected to immunoprecipitation with DYKDDDDK Fab-Trap™ and elution with 2x SDS-sample buffer. SDS-PAGE analysis shows elution of Fab-fragment only without contamination of endogenous proteins. L: Prestained protein marker (Proteintech, PL00001), I: Input, B: Bound.

    Analysis of unspecific binding of endogenous proteins to the DYKDDDDK Fab-Trap™ in the absence of Flag® fusion protein. Lysates devoid of Flag® fusion protein from HEK cells (HEK293T), Yeast (Saccharomyces cerevisiae), Insect cells (HighFive), Bacteria (Escherichia coli TG1) , and Plant (Arabidopsis thaliana) were subjected to immunoprecipitation with DYKDDDDK Fab-Trap™ and elution with 2x SDS-sample buffer. SDS-PAGE analysis shows elution of Fab-fragment only without contamination of endogenous proteins. L: Prestained protein marker (Proteintech, PL00001), I: Input, B: Bound.

Product Information

The ChromoTek DYKDDDDK Fab-Trap™ Agarose consists of an anti-DYKDDDDK-tag Fab-fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap™ Agarose is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

DescriptionThe DYKDDDDK Fab-Trap Agarose is a premium affinity resin for efficient immunoprecipitation (IP) of Flag®-tagged proteins. The DYKDDDDK Fab-Trap Agarose consists of a Fab-fragment, which is covalently bound to agarose beads.

• Lowest background in class

• Less contaminating antibody chains

• Recombinantly expressed Fab-fragment derived from M2 clone

• Compatible with harsh buffer conditions

• Ready-to-use

ApplicationsIP, CoIP, ChIP, RIP, Protein purification
Specificity/TargetThe DYKDDDDK Fab-Trap™ Agarose specifically binds to 1x and 3xDYKDDDDK-tag sequence (1x and 3xFlag®-tag). It binds to same epitope as Sigma's Anti-FLAG® M2 Antibody and is compatible with N-, C-terminal or internal tagging.
Binding capacity12.5 - 15 µg of recombinant 3xDYKDDDDK-tagged protein (~30 kDa) per 25µL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution buffer3xDYKDDDDK-peptide
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility0.5 % Nonidet P40 Substitute, 5 % Tween™ 20, 2 M NaCl, 10 mM TCEP, 10 mM DTT, 10 mM ß-mercaptoethanol, 2 M Urea
Type Fab-fragment
ClassRecombinant
HostMouse
RRIDAB_2894836
Storage Buffer1x PBS, Preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
IP

J Mol Biol

The integration of proteome-wide PTM data with protein structural and sequence features identifies phosphorylations that mediate 14-3-3 interactions

Authors - Ce Egbert
View Article
IP

Cell Rep

SPATA2 restricts OTULIN-dependent LUBAC activity independently of CYLD

Authors - Laura Griewahn
View Article

J Virol

Pseudorabies Virus Regulates the Extracellular Translocation of Annexin A2 To Promote Its Proliferation

Authors - Maoyang Weng
View Article
CoIP

PLoS Pathog

The alphaherpesvirus conserved pUS10 is important for natural infection and its expression is regulated by the conserved Herpesviridae protein kinase (CHPK)

Authors - Nagendraprabhu Ponnuraj
View Article
IP

Curr Biol

Conservation of the PBL-RBOH immune module in land plants

Authors - Jiashu Chu
View Article

bioRxiv

Characterization of human XPD helicase activity with Single Molecule Magnetic Tweezers

Authors - Chunfeng Mao
View Article