验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HT-1080 cells, HeLa cells, L02 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20456-1-AP targets EI24 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | EI24 fusion protein Ag14169 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | etoposide induced 2.4 mRNA |
| Synonyms | EI24, etoposide induced 2.4 mRNA, p53 induced gene 8 protein, p53-induced factor Ei24, PIG8, TP53I8 |
| Calculated Molecular Weight | 340 aa, 39 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC002390 |
| Gene Symbol | EI24 |
| Gene ID (NCBI) | 9538 |
| RRID | AB_10858326 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14681 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ei24 is an ER-localized protein originally identified as a p53-induced proapoptotic gene in etoposide-treated NIH3T3 cells. It has been shown to be able to bind to the antiapoptotic protein Bcl-2 (PMID: 15781622), play a role in autophagy (PMID: 23074225), and induce proliferate arrest/apoptosis (PMID: 10594026). Ei24 can bind specifically to IMPβ1 and IMPα2 to impede their normal role in nuclear import (PMID: 24821838).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EI24 antibody 20456-1-AP | Download protocol |
| IHC protocol for EI24 antibody 20456-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biochem Cell Biol Etoposide-induced protein 2.4 ameliorates high glucose-induced epithelial-mesenchymal transition by activating adenosine monophosphate-activated protein kinase pathway in renal tubular cells
| ||
BMC Cancer Circular RNA hsa_circ_0043278 inhibits breast cancer progression via the miR-455-3p/EI24 signalling pathway
| ||
Expert Rev Mol Diagn Prognostic role and immune infiltration characteristics of EI24 in multiple cancer types |
