验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Jurkat cells, mouse thymus tissue, HepG2 cells, HeLa cells, rat heart tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, THP-1 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy EIF2AK2 Ready-To-Use IHC Kit. EIF2AK2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18244-1-AP targets EIF2AK2, PKR in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig, rabbit, goat |
| 免疫原 | EIF2AK2, PKR fusion protein Ag13010 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 2-alpha kinase 2 |
| 别名 | EIF2AK2, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2 |
| 计算分子量 | 551 aa, 62 kDa |
| 观测分子量 | 65-68 kDa |
| GenBank蛋白编号 | BC093676 |
| 基因名称 | EIF2AK2 |
| Gene ID (NCBI) | 5610 |
| RRID | AB_2246451 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P19525 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF2AK2, PKR antibody 18244-1-AP | Download protocol |
| IHC protocol for EIF2AK2, PKR antibody 18244-1-AP | Download protocol |
| IF protocol for EIF2AK2, PKR antibody 18244-1-AP | Download protocol |
| IP protocol for EIF2AK2, PKR antibody 18244-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors. | ||
Nat Neurosci Maternal immune activation in mice disrupts proteostasis in the fetal brain. | ||
Sci Adv Temporal control of the integrated stress response by a stochastic molecular switch. | ||
Nat Commun NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice. | ||
J Immunother Cancer Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironment | ||
