验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10686-1-AP targets AGO2 in WB, IP, RIP, ELISA, CLIP applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP, RIP, CLIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, fish |
| 免疫原 | AGO2 fusion protein Ag1032 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 2C, 2 |
| 别名 | AGO2, Argonaute2, eIF 2C 2, eIF2C 2, EIF2C2, hAgo2, PAZ Piwi domain protein, PPD, Protein argonaute 2, Protein slicer, Q10 |
| 计算分子量 | 97 kDa |
| 观测分子量 | 94-97 kDa |
| GenBank蛋白编号 | BC007633 |
| 基因名称 | EIF2C2 |
| Gene ID (NCBI) | 27161 |
| RRID | AB_2096299 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UKV8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EIF2C2, also named as AGO2, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). EIF2C2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. It also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. EIF2C2 can also upregulate the translation of specific mRNAs under certain growth conditions. This antibody is specific to EIF2C2. EIF2C2 exists two isoforms and molecular weight of isoforms are 97 kDa and 94 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AGO2 antibody 10686-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Liver governs adipose remodelling via extracellular vesicles in response to lipid overload. | ||
Dev Cell Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia. | ||
J Hematol Oncol LINC01123, a c-Myc-activated long non-coding RNA, promotes proliferation and aerobic glycolysis of non-small cell lung cancer through miR-199a-5p/c-Myc axis. | ||
Clin Transl Med LncRNA nuclear-enriched abundant transcript 1 shuttled by prostate cancer cells-secreted exosomes initiates osteoblastic phenotypes in the bone metastatic microenvironment via miR-205-5p/runt-related transcription factor 2/splicing factor proline- and glutamine-rich/polypyrimidine tract-binding protein 2 axis. |