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EIF3D Monoclonal antibody, PBS Only

EIF3D Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2C5A3

Cat No : 66024-1-PBS

Print datasheet

Synonyms

eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7

验证数据展示

  • WB analysis of HepG2 using 66024-1-Ig (same clone as 66024-1-PBS)

    WB analysis of HepG2 using 66024-1-Ig (same clone as 66024-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 66024-1-Ig (same clone as 66024-1-PBS)

    IHC staining of human brain using 66024-1-Ig (same clone as 66024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF3D antibody included
  • IHC staining of human brain using 66024-1-Ig (same clone as 66024-1-PBS)

    IHC staining of human brain using 66024-1-Ig (same clone as 66024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF3D antibody included
  • IF Staining of HeLa using 66024-1-Ig (same clone as 66024-1-PBS)

    IF Staining of HeLa using 66024-1-Ig (same clone as 66024-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66024-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EIF3D Ready-To-Use IHC Kit. EIF3D primary antibody included.

产品信息

66024-1-PBS targets EIF3D in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse, rat
Immunogen EIF3D fusion protein Ag18092 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit D
Synonyms eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC000328
Gene Symbol EIF3D
Gene ID (NCBI) 8664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15371
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.