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- KD/KO Validated
EIF3M Polyclonal antibody
EIF3M Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy EIF3M Ready-To-Use IHC Kit. EIF3M primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11423-1-AP targets EIF3M in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, CoIP, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | EIF3M fusion protein Ag1994 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | eukaryotic translation initiation factor 3, subunit M |
| Synonyms | PCID1, hFL-B5, HFLB5, hfl B5, GA17 |
| Calculated Molecular Weight | 374 aa, 43 kDa |
| Observed Molecular Weight | 35-43 kDa |
| GenBank Accession Number | BC019103 |
| Gene Symbol | EIF3M |
| Gene ID (NCBI) | 10480 |
| RRID | AB_2246386 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7L2H7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF3M antibody 11423-1-AP | Download protocol |
| IHC protocol for EIF3M antibody 11423-1-AP | Download protocol |
| IF protocol for EIF3M antibody 11423-1-AP | Download protocol |
| IP protocol for EIF3M antibody 11423-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine. | ||
Oncogene eIF3m expression influences the regulation of tumorigenesis-related genes in human colon cancer.
| ||
Am J Cancer Res EIF3m promotes the malignant phenotype of lung adenocarcinoma by the up-regulation of oncogene CAPRIN1.
| ||
iScience Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges |
