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EIF4B Polyclonal antibody

EIF4B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

Conjugate

Unconjugated

Cat no : 17917-1-AP

Print datasheet

Synonyms

PRO1843, eIF-4B, EIF 4B



经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, sodium arsenite treated HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17917-1-AP targets EIF4B in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen EIF4B fusion protein Ag12325 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4B
Synonyms PRO1843, eIF-4B, EIF 4B
Calculated Molecular Weight 611 aa, 69 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC073154
Gene Symbol EIF4B
Gene ID (NCBI) 1975
RRIDAB_2097541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23588
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623).

实验方案

Product Specific Protocols
WB protocol for EIF4B antibody 17917-1-APDownload protocol
IHC protocol for EIF4B antibody 17917-1-APDownload protocol
IF protocol for EIF4B antibody 17917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell

Circadian clocks are modulated by compartmentalized oscillating translation

Authors - Yanrong Zhuang
humanWB

Brain

MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.

Authors - Bing-Lin Zhu
ratWB

Nucleic Acids Res

PMID: 30295850

Authors - Zonghong Li
WB

Mol Med Rep

MicroRNA‑216a inhibits the growth and metastasis of oral squamous cell carcinoma by targeting eukaryotic translation initiation factor 4B.

Authors - Lei Li
humanWB

Biotechnol J

Highly efficient identification of nucleocytoplasmic O-glycosylation by the TurboID-based proximity labeling method in living cells

Authors - Yunfeng Long
humanWB,IF,CoIP

Biochem Pharmacol

Phytic acid promotes high glucose-mediated bone marrow mesenchymal stem cells osteogenesis via modulating circEIF4B that sponges miR-186-5p and complexes with IGF2BP3

Authors - Jin Wu