• Featured Product
  • KD/KO验证

Emerin Polyclonal antibody

Emerin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10351-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

EMD, STA, LEMD5, EDMD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, COLO 320 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293T cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10351-1-AP targets Emerin in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 Emerin fusion protein Ag0236 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 emerin
别名 EMD, STA, LEMD5, EDMD
计算分子量 34 kDa
观测分子量 35 kDa
GenBank蛋白编号BC000738
基因名称 EMD
Gene ID (NCBI) 2010
RRIDAB_2100056
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50402
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

实验方案

Product Specific Protocols
WB protocol for Emerin antibody 10351-1-APDownload protocol
IHC protocol for Emerin antibody 10351-1-APDownload protocol
IF protocol for Emerin antibody 10351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nature

Cytoplasmic control of intranuclear polarity by human cytomegalovirus.

Authors - Dean J Procter
humanIF

Nature

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Authors - Shiwei Liu
IF

Cell

DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.

Authors - Matthias Samwer
humanIF,IP

Nat Commun

Off-the-shelf proximity biotinylation for interaction proteomics.

Authors - Irene Santos-Barriopedro
humanIF

Dev Cell

Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.

Authors - Holly Merta
humanIF

J Cell Biol

Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation.

Authors - Banafsheh Golchoubian
Loading...