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EMX1 Polyclonal antibody

EMX1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55032-1-AP

Print datasheet

Synonyms

Empty spiracles-like protein 1

验证数据展示

  • WB analysis of mouse brain using 55032-1-AP

    WB analysis of mouse brain using 55032-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55032-1-AP (EMX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 55032-1-AP

    IHC staining of rat kidney using 55032-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 55032-1-AP (EMX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EMX1 antibody included
  • IHC staining of rat kidney using 55032-1-AP

    IHC staining of rat kidney using 55032-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 55032-1-AP (EMX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EMX1 antibody included

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EMX1 Ready-To-Use IHC Kit. EMX1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55032-1-AP targets EMX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name empty spiracles homeobox 1
Synonyms Empty spiracles-like protein 1
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberNM_004097
Gene Symbol EMX1
Gene ID (NCBI) 2016
RRIDAB_2881259
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ04741
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EMX1 antibody 55032-1-APDownload protocol
IHC protocol for EMX1 antibody 55032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols