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验证数据展示

  • IF Staining of MCF-7 using CL594-66316

    IF Staining of MCF-7 using CL594-66316

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 EPCAM/CD326 antibody (CL594-66316, Clone: 2A2D5 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated EPCAM/CD326 Monoclonal antibody

EPCAM/CD326 Monoclonal Antibody for IF/ICC
Cat No. CL594-66316

Print datasheet

CloneNo. 2A2D5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Synonyms

EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy EPCAM/CD326 Ready-To-Use IHC Kit. EPCAM/CD326 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66316-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66316 targets EPCAM/CD326 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen EPCAM/CD326 fusion protein Ag15393 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name epithelial cell adhesion molecule
Synonyms EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC014785
Gene Symbol EPCAM
Gene ID (NCBI) 4072
RRIDAB_2883555
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16422
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

实验方案

Product Specific Protocols
IF protocol for CL594 EPCAM/CD326 antibody CL594-66316Download protocol
Standard Protocols
Click here to view our Standard Protocols