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验证数据展示

  • WB analysis of A431 using 66316-1-Ig (same clone as 66316-1-PBS)

    WB analysis of A431 using 66316-1-Ig (same clone as 66316-1-PBS)

    WB result of EPCAM antibody (66316-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • WB analysis using 66316-1-Ig (same clone as 66316-1-PBS)

    WB analysis using 66316-1-Ig (same clone as 66316-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66316-1-Ig (EPCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • IHC staining of Human colon using 66316-1-Ig (same clone as 66316-1-PBS)

    IHC staining of Human colon using 66316-1-Ig (same clone as 66316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue slide using 66316-1-Ig (EPCAM/CD326 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EPCAM/CD326 antibody included
  • IHC staining of human breast cancer using 66316-1-Ig (same clone as 66316-1-PBS)

    IHC staining of human breast cancer using 66316-1-Ig (same clone as 66316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66316-1-Ig (EPCAM Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EPCAM/CD326 antibody included
  • IF Staining of MCF-7 using 66316-1-Ig (same clone as 66316-1-PBS)

    IF Staining of MCF-7 using 66316-1-Ig (same clone as 66316-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EPCAM antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 66316-1-Ig (same clone as 66316-1-PBS)

    IF Staining of human colon cancer using 66316-1-Ig (same clone as 66316-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

EPCAM/CD326 Monoclonal antibody, PBS Only

EPCAM/CD326 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66316-1-PBS

产品说明书

CloneNo. 2A2D5

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EPCAM/CD326 Ready-To-Use IHC Kit. EPCAM/CD326 primary antibody included.

产品信息

66316-1-PBS targets EPCAM/CD326 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 EPCAM/CD326 fusion protein Ag15393 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 epithelial cell adhesion molecule
别名 EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326
计算分子量 314 aa, 35 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC014785
基因名称 EPCAM
Gene ID (NCBI) 4072
RRIDAB_2881697
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16422
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)