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  • Featured Product
  • KD/KO Validated

EPCAM/CD326 Monoclonal antibody, PBS Only

EPCAM/CD326 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2A2D5

Cat no : 66316-1-PBS

Print datasheet

Synonyms

EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326

验证数据展示

  • WB analysis of A431 using 66316-1-Ig (same clone as 66316-1-PBS)

    WB analysis of A431 using 66316-1-Ig (same clone as 66316-1-PBS)

    WB result of EPCAM antibody (66316-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • WB analysis using 66316-1-Ig (same clone as 66316-1-PBS)

    WB analysis using 66316-1-Ig (same clone as 66316-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66316-1-Ig (EPCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66316-1-Ig (same clone as 66316-1-PBS)

    IHC staining of human breast cancer using 66316-1-Ig (same clone as 66316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66316-1-Ig (EPCAM Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EPCAM/CD326 antibody included
  • IF Staining of MCF-7 using 66316-1-Ig (same clone as 66316-1-PBS)

    IF Staining of MCF-7 using 66316-1-Ig (same clone as 66316-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EPCAM antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 66316-1-Ig (same clone as 66316-1-PBS)

    IF Staining of human colon cancer using 66316-1-Ig (same clone as 66316-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EPCAM/CD326 Ready-To-Use IHC Kit. EPCAM/CD326 primary antibody included.

产品信息

66316-1-PBS targets EPCAM/CD326 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen EPCAM/CD326 fusion protein Ag15393 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name epithelial cell adhesion molecule
Synonyms EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC014785
Gene Symbol EPCAM
Gene ID (NCBI) 4072
RRIDAB_2881697
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16422
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)