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ERK1/2 Monoclonal antibody, PBS Only

ERK1/2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G8A11

Cat no : 66192-1-PBS

Print datasheet

Synonyms

ERK, ERK 1, ERK 2, ERK1, ERK1/2, ERK2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

验证数据展示

  • WB analysis using 66192-1-Ig (same clone as 66192-1-PBS)

    WB analysis using 66192-1-Ig (same clone as 66192-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

    IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

    IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66192-1-Ig (same clone as 66192-1-PBS)

    IF Staining of HeLa using 66192-1-Ig (same clone as 66192-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ERK1/2 antibody (66192-1-Ig, Clone: 4G8A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

产品信息

66192-1-PBS targets ERK1/2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen ERK1/2 fusion protein Ag20350 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitogen-activated protein kinase 3
Synonyms ERK, ERK 1, ERK 2, ERK1, ERK1/2, ERK2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 38-44 kDa
GenBank Accession NumberBC013992
Gene Symbol ERK1
Gene ID (NCBI) 5595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27361
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.