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验证数据展示

  • WB analysis using 66192-1-Ig (same clone as 66192-1-PBS)

    WB analysis using 66192-1-Ig (same clone as 66192-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

    IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

    IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66192-1-Ig (same clone as 66192-1-PBS)

    IF Staining of HeLa using 66192-1-Ig (same clone as 66192-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ERK1/2 antibody (66192-1-Ig, Clone: 4G8A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

ERK1/2 Monoclonal antibody, PBS Only

ERK1/2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66192-1-PBS

产品说明书

CloneNo. 4G8A11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ERK1, ERK, ERK2, ERK-1, EC:2.7.11.24

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66192-1-PBS targets ERK1/2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 ERK1/2 fusion protein Ag20350 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 3
别名 ERK1, ERK, ERK2, ERK-1, EC:2.7.11.24
计算分子量 43 kDa
观测分子量 38-44 kDa
GenBank蛋白编号BC013992
基因名称 ERK1
Gene ID (NCBI) 5595
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP27361
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.