• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

EYA4 Polyclonal antibody

EYA4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24691-1-AP

Print datasheet

Synonyms

CMD1J, DFNA10, EYA4, Eyes absent homolog 4

验证数据展示

  • WB analysis using 24691-1-AP

    WB analysis using 24691-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24691-1-AP (EYA4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 24691-1-AP

    IHC staining of human skeletal muscle using 24691-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24691-1-AP (EYA4 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with EYA4 antibody included
  • IHC staining of human skeletal muscle using 24691-1-AP

    IHC staining of human skeletal muscle using 24691-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24691-1-AP (EYA4 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with EYA4 antibody included
  • IF Staining of HeLa using 24691-1-AP

    IF Staining of HeLa using 24691-1-AP

    Immunofluorescent analysis of HeLa cells using 24691-1-AP (EYA4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue, L02 cells, rat liver tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EYA4 Ready-To-Use IHC Kit. EYA4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24691-1-AP targets EYA4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen EYA4 fusion protein Ag20466 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eyes absent homolog 4 (Drosophila)
Synonyms CMD1J, DFNA10, EYA4, Eyes absent homolog 4
Calculated Molecular Weight 639 aa, 70 kDa
Observed Molecular Weight 67-70 kDa
GenBank Accession NumberBC041063
Gene Symbol EYA4
Gene ID (NCBI) 2070
RRIDAB_2879675
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95677
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EYA4 (Eyes absent homolog 4) participates directly in the modulation of cellular signaling through its phosphatase activity and plays a role in the embryonic and post-natal development of the mature organs of CORTI. This protein may be involved in the development of the eye. It has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for EYA4 antibody 24691-1-APDownload protocol
IHC protocol for EYA4 antibody 24691-1-APDownload protocol
IF protocol for EYA4 antibody 24691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cell Res

Lin28a maintains a subset of adult muscle stem cells in an embryonic-like state

Authors - Peng Wang
View Article
humanWB,IHC

Signal Transduct Target Ther

Phosphorylation determines the glucose metabolism reprogramming and tumor-promoting activity of sine oculis homeobox 1

Authors - Yanni Lin
View Article