验证数据展示
经过测试的应用
Positive WB detected in | human plasma tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66509-1-Ig targets Coagulation Factor II in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Coagulation Factor II fusion protein Ag18385 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | coagulation factor II (prothrombin) |
Synonyms | Prothrombin, F2, EC:3.4.21.5, Activation peptide fragment 2, Activation peptide fragment 1 |
Calculated Molecular Weight | 622 aa, 70 kDa |
Observed Molecular Weight | 70-80 kDa |
GenBank Accession Number | BC051332 |
Gene Symbol | F2 |
Gene ID (NCBI) | 2147 |
RRID | AB_2881872 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P00734 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II, also known as prothrombin or factor II, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.
实验方案
Product Specific Protocols | |
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WB protocol for Coagulation Factor II antibody 66509-1-Ig | Download protocol |
IHC protocol for Coagulation Factor II antibody 66509-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |