验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, human brain tissue, mouse adipose tissue |
| Positive IHC detected in | human malignant melanoma tissue, human breast cancer tissue, human gliomas tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51010-1-AP targets FABP7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | fatty acid binding protein 7, brain |
| Synonyms | B FABP, BLBP, Brain lipid binding protein, FABP7, FABPB, Fatty acid binding protein 7, MRG |
| Calculated Molecular Weight | 132aa,15 kDa; 166aa,19 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC012299 |
| Gene Symbol | FABP7 |
| Gene ID (NCBI) | 2173 |
| RRID | AB_2100456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15540 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FABP7 (brain fatty acid-binding protein; BLBP) is a member of the FABP family of lipid chaperones involved in the uptake and intracellular trafficking of fatty acids. FABP7 is expressed in radial glial cells during brain development. FABP7 is up-regulated in brain tumor tissue; its expression has been associated with decreased survival times and tumor progression in patients with astrocytoma, melanoma, basal-type breast cancer, and renal cell carcinoma. This antibody is useful to label radial gial cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FABP7 antibody 51010-1-AP | Download protocol |
| IHC protocol for FABP7 antibody 51010-1-AP | Download protocol |
| IF protocol for FABP7 antibody 51010-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Nano Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy | ||
Biomaterials MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice. | ||
Nat Commun Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. | ||
Cancer Res Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer. | ||
Int J Biol Macromol Soybean soluble polysaccharide prevents obesity in high-fat diet-induced rats via lipid metabolism regulation | ||
Front Immunol RGC-32 Regulates Generation of Reactive Astrocytes in Experimental Autoimmune Encephalomyelitis. |
