验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, SGC-7901 cells, hTERT-RPE1 cells |
| Positive IHC detected in | human skin tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15939-1-AP targets Piezo1 (extracellular domain) in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig, canine, zebrafish |
| 免疫原 | Piezo1 (extracellular domain) fusion protein Ag7791 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 38, member A |
| 别名 | FAM38A, PIEZO1, Protein FAM38A, Piezo-type mechanosensitive ion channel component 1, Membrane protein induced by beta-amyloid treatment |
| 计算分子量 | 286 kDa |
| 观测分子量 | 233-286 kDa |
| GenBank蛋白编号 | BC008073 |
| 基因名称 | Piezo1/FAM38A |
| Gene ID (NCBI) | 9780 |
| RRID | AB_2231460 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92508 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Piezo1 (extracellular domain) antibody 15939-1-AP | Download protocol |
| IHC protocol for Piezo1 (extracellular domain) antibody 15939-1-AP | Download protocol |
| IF protocol for Piezo1 (extracellular domain) antibody 15939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature Skin cells undergo asynthetic fission to expand body surfaces in zebrafish.
| ||
Nature Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia.
| ||
Nature Mechanical stretch triggers rapid epithelial cell division through Piezo1.
| ||
Blood Mechanosensation by endothelial PIEZO1 is required for leukocyte diapedesis.
|
