• Featured Product
  • KD/KO Validated

FEM1B Polyclonal antibody

FEM1B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 19544-1-AP

Print datasheet

Synonyms

F1A alpha, F1AA, fem 1 homolog b (C. elegans), FEM1 beta, FEM1B, FIAA, KIAA0396, Protein fem 1 homolog B



经过测试的应用

Positive WB detected inPC-3 cells, mouse pancreas tissue, mouse testis tissue
Positive IHC detected inhuman pancreas cancer tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19544-1-AP targets FEM1B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fem-1 homolog b (C. elegans)
Synonyms F1A alpha, F1AA, fem 1 homolog b (C. elegans), FEM1 beta, FEM1B, FIAA, KIAA0396, Protein fem 1 homolog B
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberNM_015322
Gene Symbol FEM1B
Gene ID (NCBI) 10116
RRIDAB_10644294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UK73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FEM1B, also named F1AA and KIAA0396, belongs to the fem-1 family. FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. It is involved in glucose homeostasis in pancreatic islets.

实验方案

Product Specific Protocols
WB protocol for FEM1B antibody 19544-1-APDownload protocol
IHC protocol for FEM1B antibody 19544-1-APDownload protocol
IF protocol for FEM1B antibody 19544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

A Cellular Mechanism to Detect and Alleviate Reductive Stress.

Authors - Andrew G Manford
  • KD Validated
humanWB

J Am Chem Soc

Discovery of a Covalent FEM1B Recruiter for Targeted Protein Degradation Applications

Authors - Nathaniel J. Henning