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验证数据展示

  • WB analysis of HEK-293 using 10060-1-AP (same clone as 10060-1-PBS)

    WB analysis of HEK-293 using 10060-1-AP (same clone as 10060-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 10060-1-AP (same clone as 10060-1-PBS)

    IP experiment of MCF-7 using 10060-1-AP (same clone as 10060-1-PBS)

    IP result of anti-FKBPL (IP:10060-1-AP, 3ug; Detection:10060-1-AP 1:800) with MCF-7 cells lysate 2500ug. This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10060-1-AP (same clone as 10060-1-PBS)

    IHC staining of human breast cancer using 10060-1-AP (same clone as 10060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10060-1-AP (same clone as 10060-1-PBS)

    IHC staining of human breast cancer using 10060-1-AP (same clone as 10060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 10060-1-AP (same clone as 10060-1-PBS)

    FC experiment of HeLa using 10060-1-AP (same clone as 10060-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug FKBPL Polyclonal antibody (10060-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FKBPL Polyclonal antibody, PBS Only

FKBPL Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA
Cat No. 10060-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), IP, ELISA

别名

DIR1, FK506-binding protein-like, NG7, WAF-1/CIP1 stabilizing protein 39, WISp39

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

10060-1-PBS targets FKBPL in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 FKBPL fusion protein Ag0112 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FK506 binding protein like
别名 DIR1, FK506-binding protein-like, NG7, WAF-1/CIP1 stabilizing protein 39, WISp39
计算分子量 349 aa, 38 kDa
观测分子量 42-48 kDa
GenBank蛋白编号BC004168
基因名称 FKBPL
Gene ID (NCBI) 63943
RRIDAB_2262677
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UIM3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193). It can be detected the band between 38 kDa and 48 kDa by western blot.