- Featured Product
- KD/KO Validated
Flotillin 1 Polyclonal antibody
Flotillin 1 Polyclonal Antibody for FC, IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, U-251 cells, mouse heart tissue, rat brain tissue, rat heart tissue, U-87 MG cells |
| Positive IP detected in | mouse brain tissue |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.05 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15571-1-AP targets Flotillin 1 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF/ICC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Flotillin 1 fusion protein Ag7902 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | flotillin 1 |
| Synonyms | FLOT1, flotillin 1 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC001146 |
| Gene Symbol | FLOT1 |
| Gene ID (NCBI) | 10211 |
| RRID | AB_2106746 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75955 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| IF protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| IP protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| FC protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. | ||
Theranostics Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization | ||
Cell Res Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death. | ||
Int J Biol Sci FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling
| ||
Cell Death Dis Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats |
