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Flotillin 1 Polyclonal antibody

Flotillin 1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15571-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (2)

FLOT1, Flotillin-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, U-251 cells, mouse heart tissue, rat brain tissue, rat heart tissue, U-87 MG cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FLOT1 Ready-To-Use IHC Kit. FLOT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15571-1-AP targets Flotillin 1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Flotillin 1 fusion protein Ag7902 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 flotillin 1
别名 FLOT1, Flotillin-1
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC001146
基因名称 Flotillin 1
Gene ID (NCBI) 10211
RRIDAB_2106746
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75955
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

实验方案

Product Specific Protocols
WB protocol for Flotillin 1 antibody 15571-1-APDownload protocol
IHC protocol for Flotillin 1 antibody 15571-1-APDownload protocol
IP protocol for Flotillin 1 antibody 15571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Authors - Xiaohan Gao
humanWB

Brain

Extracellular vesicle biomarkers for complement dysfunction in schizophrenia

Authors - Ting Xue
human,mouseWB

Theranostics

Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization

Authors - Yuting Lin
mouseWB

Cell Res

Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death.

Authors - Xin Chen
human,mouseWB,IHC

Int J Biol Sci

FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling

Authors - Ran Wang
  • KD Validated
ratWB

Cell Death Dis

Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats

Authors - Yajie Wang
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