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- KD/KO Validated
c-Fos Polyclonal antibody
c-Fos Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | RAW 264.7 cells |
Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 26192-1-AP is c-Fos Fusion Protein expressed in E. coli.
Tested Applications | WB, IF, IHC, ELISA Application Description |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, canine |
Immunogen | c-Fos fusion protein Ag24340 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | FOS |
Synonyms | AP 1, C FOS, Cellular oncogene fos, c-Fos, FOS, G0S7, Proto oncogene c Fos |
Calculated Molecular Weight | 41 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC004490 |
Gene Symbol | c-Fos |
Gene ID (NCBI) | 2353 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P01100 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for c-Fos antibody 26192-1-AP | Download protocol |
IHC protocol for c-Fos antibody 26192-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. | ||
Nat Commun Ketamine activates adult-born immature granule neurons to rapidly alleviate depression-like behaviors in mice. | ||
Biol Psychiatry Fighting Females: Neural and Behavioral Consequences of Social Defeat Stress in Female Mice. | ||