验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, A549 cells, HepG2 cells, MDA-MB-231 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat stomach tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22474-1-AP targets FOXA2 in WB, IHC, IF-P, IP, CoIP, chIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, chIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | FOXA2 fusion protein Ag18221 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | forkhead box A2 |
| 别名 | HNF3B, HNF 3B, HNF 3 beta, Hepatocyte nuclear factor 3-beta, Forkhead box protein A2 |
| 计算分子量 | 457 aa, 48 kDa |
| 观测分子量 | 55-65 kDa |
| GenBank蛋白编号 | BC011780 |
| 基因名称 | FOXA2 |
| Gene ID (NCBI) | 3170 |
| RRID | AB_2879110 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y261 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon INS stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXA2 antibody 22474-1-AP | Download protocol |
| IHC protocol for FOXA2 antibody 22474-1-AP | Download protocol |
| IF protocol for FOXA2 antibody 22474-1-AP | Download protocol |
| IP protocol for FOXA2 antibody 22474-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer. | ||
iScience NatD epigenetically activates FOXA2 expression to promote breast cancer progression by facilitating MMP14 expression | ||
Chemosphere Comparative cytotoxicity studies of halophenolic disinfection byproducts using human extended pluripotent stem cells. | ||
Am J Cancer Res Epigenetically silenced linc00261 contributes to the metastasis of hepatocellular carcinoma via inducing the deficiency of FOXA2 transcription.
| ||
Int J Biol Sci EGF suppresses the expression of miR-124a in pancreatic β cell lines via ETS2 activation through the MEK and PI3K signaling pathways. | ||
Mol Cell Endocrinol Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells. |