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Fascin Polyclonal antibody
Fascin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF-P, FC (Intra), ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse thymus tissue, K-562 cells, NIH/3T3 cells, SH-SY5Y cells |
Positive IHC detected in | human tonsillitis tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive FC (Intra) detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14384-1-AP targets Fascin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | Fascin fusion protein Ag5816 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus) |
Synonyms | FSCN1, HSN, Fascin 1,Fascin, FAN1, 55 kDa actin-bundling protein |
Calculated Molecular Weight | 55 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC000521 |
Gene Symbol | Fascin |
Gene ID (NCBI) | 6624 |
ENSEMBL Gene ID | ENSG00000075618 |
RRID | AB_2106381 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q16658 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Fascin antibody 14384-1-AP | Download protocol |
IHC protocol for Fascin antibody 14384-1-AP | Download protocol |
IF protocol for Fascin antibody 14384-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Aging (Albany NY) Exosomal lncRNA LINC01711 facilitates metastasis of esophageal squamous cell carcinoma via the miR-326/FSCN1 axis. | ||
Int J Biol Macromol Recombinant porcine NK-lysin inhibits the invasion of hepatocellular carcinoma cells in vitro. | ||
Exp Cell Res Downregulation of fascin in the first trimester placental villi is associated with early recurrent miscarriage.
| ||
J Exp Clin Cancer Res The role of microRNA-133b and its target gene FSCN1 in gastric cancer. | ||
Immunol Lett Yes-associated protein upregulates filopodia formation to promote alveolar epithelial-cell phagocytosis.
| ||
J Anat Localization of macrophages and dendritic cells in human thoracic lymph nodes: An immunohistochemical study using surgically obtained specimens |