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FUS/TLS Monoclonal antibody
FUS/TLS Monoclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig and More (1)
Applications
WB, IP, IF, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3A10B5
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HeLa cells, HL-60 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human gliomas tissue, human colon tissue, human brain (FTLD) tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60160-1-Ig targets FUS/TLS in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, IP, IF, IHC, ELISA Application Description |
Cited Applications | WB, IP, IF, RIP, IHC |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, Drosophila |
Immunogen | FUS/TLS fusion protein Ag2150 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | fusion (involved in t(12;16) in malignant liposarcoma) |
Synonyms | 75 kDa DNA pairing protein, FUS, FUS CHOP, FUS1, FUS-CHOP, hnRNP P2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, TLS, TLS/CHOP |
Calculated Molecular Weight | 75 kDa |
Observed Molecular Weight | 68-75 kDa |
GenBank Accession Number | BC026062 |
Gene Symbol | FUS/TLS |
Gene ID (NCBI) | 2521 |
RRID | AB_10666169 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P35637 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for FUS/TLS antibody 60160-1-Ig | Download protocol |
IHC protocol for FUS/TLS antibody 60160-1-Ig | Download protocol |
IF protocol for FUS/TLS antibody 60160-1-Ig | Download protocol |
IP protocol for FUS/TLS antibody 60160-1-Ig | Download protocol |
FC protocol for FUS/TLS antibody 60160-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Cell Biol The RNA-binding protein Fus directs translation of localized mRNAs in APC-RNP granules.
| ||
Brain Motor neuron degeneration in spastic paraplegia 11 mimics amyotrophic lateral sclerosis lesions. | ||
Acta Neuropathol Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations. | ||
Cell Rep A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma | ||
Acta Neuropathol Commun RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases. |