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Frizzled 8 Polyclonal antibody

Frizzled 8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55093-1-AP

Print datasheet

Synonyms

Frizzled 8, FZ 8, FZD8, hFZ8

验证数据展示

  • WB analysis of HeLa using 55093-1-AP

    WB analysis of HeLa using 55093-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 55093-1-AP

    IHC staining of human pancreas cancer using 55093-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55093-1-AP (Frizzled 8 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 55093-1-AP

    IHC staining of human pancreas cancer using 55093-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55093-1-AP (Frizzled 8 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, mouse embryo tissue, mouse heart tissue, mouse lung tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

55093-1-AP targets Frizzled 8 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name frizzled homolog 8 (Drosophila)
Synonyms Frizzled 8, FZ 8, FZD8, hFZ8
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 70-73 kDa
GenBank Accession NumberNM_031866
Gene Symbol Frizzled 8
Gene ID (NCBI) 8325
RRIDAB_2881267
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H461
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Frizzled 8 antibody 55093-1-APDownload protocol
IHC protocol for Frizzled 8 antibody 55093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.

Authors - Wenjia Liu
View Article