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  • Featured Product
  • KD/KO Validated

Fibronectin Monoclonal antibody, PBS Only

Fibronectin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1G10F9

Cat no : 66042-1-PBS

Print datasheet

Synonyms

FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG

验证数据展示

  • WB analysis of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)

    WB analysis of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • IP experiment of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)

    IP experiment of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)

    IP result of anti-Fibronectin (IP:66042-1-Ig, 5ug; Detection:66042-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    IF Staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • WB analysis using 66042-1-Ig (same clone as 66042-1-PBS)

    WB analysis using 66042-1-Ig (same clone as 66042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • WB analysis of HuH-7 using 66042-1-Ig (same clone as 66042-1-PBS)

    WB analysis of HuH-7 using 66042-1-Ig (same clone as 66042-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 66042-1-Ig (same clone as 66042-1-PBS)

    IF Staining of NIH/3T3 using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

产品信息

66042-1-PBS targets Fibronectin in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Fibronectin fusion protein Ag8016 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fibronectin 1
Synonyms FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG
Calculated Molecular Weight 2386 aa, 263 kDa
Observed Molecular Weight 263 kDa
GenBank Accession NumberBC005858
Gene Symbol Fibronectin
Gene ID (NCBI) 2335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02751
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)