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验证数据展示

  • WB analysis of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)

    WB analysis of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • IP experiment of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)

    IP experiment of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)

    IP result of anti-Fibronectin (IP:66042-1-Ig, 5ug; Detection:66042-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    IF Staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • WB analysis using 66042-1-Ig (same clone as 66042-1-PBS)

    WB analysis using 66042-1-Ig (same clone as 66042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • WB analysis of HuH-7 using 66042-1-Ig (same clone as 66042-1-PBS)

    WB analysis of HuH-7 using 66042-1-Ig (same clone as 66042-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 66042-1-Ig (same clone as 66042-1-PBS)

    IF Staining of NIH/3T3 using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Fibronectin Monoclonal antibody, PBS Only

Fibronectin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 66042-1-PBS

产品说明书

CloneNo. 1G10F9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA

别名

FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66042-1-PBS targets Fibronectin in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Fibronectin fusion protein Ag8016 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fibronectin 1
别名 FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG
计算分子量 2386 aa, 263 kDa
观测分子量 263 kDa
GenBank蛋白编号BC005858
基因名称 Fibronectin
Gene ID (NCBI) 2335
RRIDAB_11182385
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02751
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)