验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy GAD1 Ready-To-Use IHC Kit. GAD1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10408-1-AP targets GAD1 in WB, IHC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-Fro, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | GAD1 fusion protein Ag0666 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutamate decarboxylase 1 (brain, 67kDa) |
| 别名 | GAD67, GAD 67, GAD 1, GAD, FLJ45882 |
| 计算分子量 | 67 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC002815 |
| 基因名称 | GAD1 |
| Gene ID (NCBI) | 2571 |
| RRID | AB_2107733 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99259 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GAD1 antibody 10408-1-AP | Download protocol |
| IHC protocol for GAD1 antibody 10408-1-AP | Download protocol |
| IF protocol for GAD1 antibody 10408-1-AP | Download protocol |
| IP protocol for GAD1 antibody 10408-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.
| ||
Sci Adv RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease. | ||
Sci Rep Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension. | ||
Transl Psychiatry Long non-coding RNA Neat1 regulates adaptive behavioural response to stress in mice. | ||
Front Mol Neurosci Follicle-stimulating hormone induces depression-like phenotype by affecting synaptic function | ||
Environ Toxicol Perinatal exposure to bisphenol A impairs cognitive function via the gamma-aminobutyric acid signaling pathway in male rat offspring |
