GAD65 Polyclonal antibody

GAD65 Polyclonal Antibody for WB, IP, ELISA
Cat No. 20746-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA and More (2)

GAD2, GAD-65, GAD 65, EC:4.1.1.15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:3000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20746-1-AP targets GAD65 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
别名 GAD2, GAD-65, GAD 65, EC:4.1.1.15
计算分子量 65 kDa
观测分子量 65 kDa
GenBank蛋白编号NM_000818
基因名称 GAD65
Gene ID (NCBI) 2572
RRIDAB_10949196
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ05329
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.

实验方案

Product Specific Protocols
WB protocol for GAD65 antibody 20746-1-APDownload protocol
IP protocol for GAD65 antibody 20746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Nat Cell Biol

Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.

Authors - De Huang
mouseWB

Neurobiol Stress

A sex-dependent delayed maturation of visual plasticity induced by adverse experiences in early childhood.

Authors - Yueqin Liu
mouseWB

Aging (Albany NY)

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Authors - Xiao Wu
mouseWB

Aging (Albany NY)

Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.

Authors - Dongdong Wang
mouseWB

CNS Neurosci Ther

Integrin α3 is required for high-frequency repetitive transcranial magnetic stimulation-induced glutamatergic synaptic transmission in mice with ischemia

Authors - Li Liu
ratWB

Sci Rep

Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension.

Authors - Hsin-Hung Chen
Loading...