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Galectin-1 Polyclonal antibody

Galectin-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 11858-1-AP

Print datasheet

Synonyms

GAL1, Gal 1, Beta-galactoside-binding lectin L-14-I, 14 kDa lectin, 14 kDa laminin-binding protein



经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, HCT 116 cells, NIH/3T3 cells, PC-12 cells, MCF-7 cells, U2OS cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy LGALS1 Ready-To-Use IHC Kit. LGALS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11858-1-AP targets Galectin-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Galectin-1 fusion protein Ag2432 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lectin, galactoside-binding, soluble, 1
Synonyms GAL1, Gal 1, Beta-galactoside-binding lectin L-14-I, 14 kDa lectin, 14 kDa laminin-binding protein
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC020675
Gene Symbol Galectin-1
Gene ID (NCBI) 3956
RRIDAB_2234673
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09382
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

实验方案

Product Specific Protocols
WB protocol for Galectin-1 antibody 11858-1-APDownload protocol
IHC protocol for Galectin-1 antibody 11858-1-APDownload protocol
IF protocol for Galectin-1 antibody 11858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Dev Cell

Gasdermin D maintains bone mass by rewiring the endo-lysosomal pathway of osteoclastic bone resorption

Authors - Mobai Li
humanIF

Front Med

Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell RNA-seq analysis

Authors - Weiwei Xian
humanWB

Cell Rep

Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.

Authors - Krishna S Tummala
humanWB

Mol Ther Nucleic Acids

Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.

Authors - Chen Yang
humanWB,IHC

Front Oncol

Prognostic Value and Biological Function of Galectins in Malignant Glioma.

Authors - Hongtao Zhu
mouseIHC

Biomed Pharmacother

Mesenchymal stem cells target microglia via galectin-1 production to rescue aged mice from olfactory dysfunction.

Authors - Yoojin Seo